Product Name: HRV3c protease
Cat No.: IT-000-HRV3c
Source: Recombinant protein from E.coli.
Formulation: 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.04% Tween20 and 50% glycerol.
Product Type: Enzyme
Protein: Activity: 1 U/ l µl; No detectable RNAase, DNAase or other protease activity. Definition of Activity Unit: One unit of HRV3c Protease is defined as the amount of enzyme needed to cleave >95% of 100µg of substrate protein at 4°C in 16 hours.
N-terminal 6xHis tagged HRV 3C Protease (Human Rhinovirus 3C Protease, PreScission Site)(GenBank# P03303).
Applications: The enzyme recognizes the cleavage site: Leu-Glu-Val-Leu-PheGln-/-Gly-Pro (LEVLFQ/GP). The enzyme exhibits highest activity around neutral pH at temperature ranging from 22 to 37°C.
Note: Cordingley, M. G., Register, R. B., Callahan, P. L., Garsky, V.M., and Colonno, R. J. (1989). Cleavage of Small Peptides In Vitro by Human Rhinovirus 14 3C Protease Expressed in Escherichia coli. J. Virol. 63, 5037-5045.
Unit: $120.00

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