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IMMUNE TECH

IT Highlights

Immune Tech Reagents for H7N9 Research

          Sporadic cases of human infection by H7 influenza viruses have been reported since 1979, caused by low- and high-pathogenic H7 viruses from both Eurasia and North American lineages. Although fatal case by H7 virus’s infection was rare, an outbreak in 2003 in Netherland by a H7N7 virus did result in one death. In recent years, increased frequency of the outbreaks of human infections by H7N3 and H7N7 viruses in North America, and Europe has raised caution in research community. In Asia, H7 influenza virus infection of human has never occurred until March 2013, when several confirmed cases of human infection by a novel H7N9 virus were reported in the city of Shanghai, China. More cases were confirmed ever since, which reached 96 cases with 18 deaths on 4/20/2013.

          Phylogenetic analysis of the available sequences derived from the novel H7N9 virus suggests that it is of avian origin, and likely the product of reassortment of at least 3 avian influenza viruses, of which:
              a) H7 probably from H7N3(A/duck/Zhejiang/12/2011) related viruses;
              b) N9 probably from H11N9(A/mallard/Czech Republic/13438-29K/2010) related viruses;
              c) PA, PB1, PB2, NS probably from several H9N2 viruses;
              d) NP probably from H11N9(A/mallard/Czech Republic/13438-29K/2010),or H7N9(A/wild bird/Korea/A14/2011) related viruses.

         As the leading provider of high quality research products in influenza and HIV fields, scientists at Immune Technology Corp have been actively monitoring the trends in emerging viruses as well as expanding product line for seasonal strains. We already have a battery of products in our catalogue suitable for virological and epidemiological studies of H7 strains. Upon learning the H7N9 epidemic, we quickly geared up to develop new products, anticipating broad demand on reagents needed for development large-scale rapid diagnosis tools for clinical as well as research. Two of the lead products are the Eurasia H7 Hemagglutinin (HA) ELISA Development Kit and Recombinant HA1 protein derived from the recent H7N9 strain in Shanghai.

 1. Recombinant proteins:

 Cat. No.  Description  Product Type   Application
 IT-003-0071p  HA1(A/chicken/Jalisco/CPA1/2012(H7N3))  Protein  WB standard,  Antibody  ELISA, immunogen, etc
 IT-003-0071ΔTMp  HA(ΔTM)(A/chicken/Jalisco/CPA1/2012(H7N3))  Protein  WB standard,  Antibody  ELISA, immunogen, etc
 IT-003-0072p  HA1(A/environment/California/7451/2010(H7N3))  Protein  WB standard,  Antibody  ELISA, immunogen, etc
 IT-003-0072ΔTMp  HA(ΔTM)(A/environment/California/7451/2010(H7N3))  Protein  WB standard,  Antibody  ELISA, immunogen, etc
 IT-003-0073p  HA1(H7N9)(A/Shanghai/1/2013)  Protein  WB standard,  Antibody  ELISA, immunogen, etc
 IT-003-0073ΔTMp  HA(ΔTM)(H7N9)(A/Shanghai/1/2013)  Protein  WB standard,  Antibody  ELISA, immunogen, etc
 IT-003-0074p  HA1(H7N9)(A/Shanghai/2/2013)  Protein  WB standard,  Antibody  ELISA, immunogen, etc
 IT-003-0074ΔTMp  HA(ΔTM)(H7N9)(A/Shanghai/2/2013)  Protein  WB standard,  Antibody  ELISA, immunogen, etc
 IT-003-007ΔTMp  HA(ΔTM)(H7N7)(A/England/268/96)  Protein  WB standard,  Antibody  ELISA, immunogen, etc
 IT-003-0081ΔTMp  HA(ΔTM)(H7N7)(A/Netherlands/219/03)  Protein  WB standard,  Antibody  ELISA, immunogen, etc
 IT-003-008ΔTMp  HA(H7N7)(A/chicken/Netherlands/1/03)  Protein  WB standard,  Antibody  ELISA, immunogen, etc
 IT-003-008p  HA1(H7N7)(A/chicken/Netherlands/1/03)  Protein  WB standard,  Antibody  ELISA, immunogen, etc

 2. Monoclonal and polyclonal antibodies:

 Cat. No.  Specificity  Isotype   Application
 IT-003-0073M1  HA(A/Shanghai/1/2013)(H7N9), HA (A/Shanghai/2/2013)(H7N9), H7(A/Netherlands/03)(H7N7), H7(A/Netherlands/ 219/03)(H7N7)  Mouse IgG1  WB, ELISA, IP, IF
 IT-003-0073M2  HA(A/Shanghai/1/2013)(H7N9), HA (A/Shanghai/2/ 2013)(H7N9), H7(A/Netherlands/03)(H7N7), H7(A/Netherlands/ 219/03)(H7N7)  Mouse IgG1  ELISA, IP, IF
 IT-003-0073M3  HA(A/Shanghai/1/2013)(H7N9), HA (A/Shanghai/2/ 2013)(H7N9), H7(A/Netherlands/03)(H7N7), H7(A/Netherlands/ 219/03)(H7N7)  Mouse IgG1  ELISA, IP, IF
 IT-003-0073M4  HA(A/Shanghai/1/2013)(H7N9), HA (A/Shanghai/2/ 2013)(H7N9), H7(A/Netherlands/03)(H7N7), H7(A/Netherlands/ 219/03)(H7N7)  Mouse IgG2a  ELISA, IP, IF
 IT-003-0073M5  HA(A/Shanghai/1/2013)(H7N9), HA (A/Shanghai/2/ 2013)(H7N9), H7(A/Netherlands/03)(H7N7), H7(A/Netherlands/ 219/03)(H7N7)  Mouse IgG2b  ELISA, IP, IF
 IT-003-0074M1  HA(A/Shanghai/1/2013)(H7N9), HA (A/Shanghai/2/2013)(H7N9), H7(A/Netherlands/03)(H7N7), H7(A/Netherlands/ 219/03)(H7N7); H15(A/Australia/83/00)(H15N8)  Mouse IgG2a  ELISA, IP, IF
 IT-003-0074M2  HA(A/Shanghai/1/2013)(H7N9), HA(A/Shanghai/2/2013)(H7N9), H7(A/Netherlands/03)(H7N7), H7(A/Netherlands/ 219/03)(H7N7); H8(A/pintail duck/Alberta/114/1979)(H8N4)  Mouse IgG1  ELISA, IP, IF
 IT-003-0074M3  HA(A/Shanghai/1/2013)(H7N9), HA(A/Shanghai/2/2013)(H7N9), H7(A/Netherlands/03)(H7N7), H7(A/Netherlands/219/03)(H7N7)  Mouse IgG2a  ELISA, IP, IF
 IT-003-0074M4  HA(A/Shanghai/1/2013)(H7N9), HA (A/Shanghai/2/2013)(H7N9), H7(A/Netherlands/03)(H7N7), H7(A/Netherlands/219/03)(H7N7)  Mouse IgG1  ELISA, IP, IF
 IT-003-0074M5  HA(A/Shanghai/1/2013)(H7N9), HA (A/Shanghai/2/2013)(H7N9), H7(A/Netherlands/03)(H7N7), H7(A/Netherlands/219/03)(H7N7); H8(A/pintail duck/Alberta/114/1979)(H8N4) and H15(A/Australia/83/00)(H15N8)  Mouse IgG2a  ELISA, IP, IF
 IT-003-0074M6  HA(A/Shanghai/1/2013)(H7N9), HA (A/Shanghai/2/2013)(H7N9), H7(A/Netherlands/03)(H7N7), H7(A/Netherlands/219/03)(H7N7)  Mouse IgG1  ELISA, IP, IF
 IT-003-0074M7  HA(A/Shanghai/1/2013)(H7N9), HA (A/Shanghai/2/2013)(H7N9), H7(A/Netherlands/03)(H7N7), H7(A/Netherlands/219/03)(H7N7)  Mouse IgG1  ELISA, IP, IF
 IT-003-0074M8  HA(A/Shanghai/1/2013)(H7N9), HA (A/Shanghai/2/2013)(H7N9), H7(A/Netherlands/03)(H7N7), H7(A/Netherlands/219/03)(H7N7)  Mouse IgG1  ELISA, IP, IF
 IT-003-0074M9  HA (A/Shanghai/2/2013)(H7N9), H7(A/Netherlands/219/03)(H7N7)  Mouse IgG1  ELISA, IP, IF
 IT-003-008M1  H7N7, H7N9  Mouse IgG2a  ELISA, IP, IF
 IT-003-008M2  H7(A/England/96)(H7N7), H7(A/Nethland/03)(H7N7), H7N9  Mouse IgG1  ELISA, IP, IF
 IT-003-008M3  H7(A/England/96)(H7N7), H7(A/Nethland/03)(H7N7), H7N9, H15(A/Australia/83/00)(H15N8)  Mouse IgG1  ELISA, IP, IF
 IT-003-008M4  H7(A/England/96)(H7N7), H7(A/Nethland/03)(H7N7), H7N9  Mouse IgG2a  ELISA, IP, IF
 IT-003-008M5  H7(A/England/96)(H7N7), H7(A/Nethland/03)(H7N7), H7N9  Mouse IgG2b  ELISA, IP, IF
 IT-003-008M6  H7(A/England/96)(H7N7), H7(A/Nethland/03)(H7N7), H7N9, H15(A/Australia/83/00)(H15N8)  Mouse IgG2a  WB,  ELISA, IP, IF
 IT-003-008M7  H7(A/England/96)(H7N7), H7(A/Nethland/03)(H7N7), H7N9, H15(A/Australia/83/00)(H15N8)  Mouse IgG2a  ELISA, IP, IF
 IT-003-008M8  H7(A/England/96)(H7N7), H7(A/Nethland/03)(H7N7), H7N9  Mouse IgG2a  ELISA, IP, IF
 IT-003-008M9  H7(A/England/96)(H7N7), H7(A/Nethland/03)(H7N7), H7N9, H15(A/Australia/83/00)(H15N8)  Mouse IgG1  ELISA, IP, IF
 IT-003-008M10  H7(A/England/96)(H7N7), H7(A/Nethland/03)(H7N7), H7N9, H15(A/Australia/83/00)(H15N8)  Mouse IgG2a  ELISA, IP, IF
 IT-003-008M11  Eurasia H7 specific, including H7 (A/Shanghai/2/ 2013(H7N9))  Mouse IgG1  ELISA, IP, IF
 IT-003-008M12  some Eurasia lineage H7, but not H7(A/Shanghai/2/2013(H7N9))  Mouse IgG2b  ELISA, IP, IF
 IT-003-008M13  H7 specific, including H7 (A/Shanghai/2/2013(H7N9))  Mouse IgG2b  ELISA, IP, IF
 IT-003-008M14  H7 and HA15(H15N8)(A/duck/AUS/341/1983), including H7 (A/Shanghai/2/2013(H7N9))  Mouse IgM  ELISA, IP, IF
 IT-003-008M15  H7 specific, including H7 (A/Shanghai/2/2013(H7N9))  Mouse IgG1  ELISA, IP, IF
 IT-003-008M16  H7 and HA15(H15N8)(A/duck/AUS/341/1983), including H7 (A/Shanghai/2/2013(H7N9))  Mouse IgG2a  ELISA, IP, IF
 IT-003-008M17  Eurasia H7 specific, including H7 (A/Shanghai/2/2013(H7N9))  Mouse IgG2b  ELISA, IP, IF
 IT-003-008  Anti-HA1(H7N7, H7N3, H7N9)  Rabbit IgG  WB, ELISA, IP, IF
 IT-003-0081  Anti-HA1(H7N9)(A/Shanghai/2/2013)  Rabbit IgG  WB, ELISA, etc

ELISA data for mABs against H7

 3. ELISA kit for H7 antigen and antibody detection:

 Cat. No.  Specificity  Production Name
 IT-E3Ag-H7(Eurasia)-Pan  Eurasia H7  H7(Eurasia)(Pan) Hemagglutinin ELISA Development Kit
 IT-E3Ag-H7-Pan  All H7  H7(Pan) Hemagglutinin ELISA Development Kit
 IT-E3Ab-IgG(H7N9)  Anti-HA(H7N9) Human IgG  Antibody Detection Kit for H7N9 HA Specific Human IgG
 IT-E3Ab-IgG/M(H7N9) (available upon request)  Anti-HA(H7N9) Human IgG & IgM  Antibody Detection Kit for H7N9 HA Specific Human IgG/M

ELISA data for mABs against H7


For additional information and request, please contact us at: info@immune-tech.com